Association of DEXA with EDX in Evaluation of Inorganic Bone Components After Application of Nano bone For Repair of Bone Defect

Ahmad, Nehad A; Elkilania, Naglaa

doi:10.21608/aadj.2022.267279

Association of DEXA with EDX in Evaluation of Inorganic Bone Components After Application of Nano bone For Repair of Bone Defect

Document Type : Original Article

Authors

Nehad A Ahmad ¹
Naglaa Elkilania ²

¹ Department of Oral and Dental Biology, Faculty of Dental Medicine, Al-Azhar University, Cairo (girls), Egypt.

² Department of Oral Medicine, Periodontology, Oral Diagnosis and Dental Radiology, Faculty of Dental Medicine, Al-Azhar University, Cairo (girls), Egypt.

10.21608/aadj.2022.267279

Abstract

Aim: The aim of this study is to correlate DEXA and EDX in assessment the variations in chemical composition in terms of the main elements comprising the bone mineral {calcium (Ca), and phosphorus (P)} after application of Nano bone for Repair of bone defect. Subjects and Methods: A bone drill defect was created in the right tibia (two holes in each tibia) of 48 rabbits; upper hole was left empty while the lower hole was packed with NanoBone. 24 animals were sacrificed 2 weeks after surgery; the remaining animals were sacrificed after 4 weeks. Half of the bone specimens prepared for the routine laboratory processing, histological and histomorphometric, while The remaining specimens were scanned by Dual-energy X-ray absorptiometry (DEXA) for bone mineral density measurement and then used for bone mineral composition determination by Energy Dispersive X-ray Analyses (EDX). The measurements of DEXA and EDX have been compared after that, data were submitted to ANOVA and Tukey’s test. Results: DEXA showed a strong positive correlation with EDX and histological analysis. Conclusion: Energy-dispersive X-ray microanalysis (EDX) is a useful quantitative tool for the analysis of Ca and P in bone in a non-invasive way.

Keywords